my gosh!
wah just when i thought its time for me to get going with experiments to produce good results, i forgot to add ampicilin into my cultures yesterday!!
and i only realised that today after i did the whole DNA purification step !
i looked at the heating block and realised tat i didnt thaw the AMP at all then it occurred to me that i didnt add it in! BOO!
so yes i didnt dare tell nick ! but in the end the results werent very gd soooooo doing another batch tml to see if there's really a problem with the bacteria or watever there can be
i usually have a checklist with me as i go thru each step but this time round i even forgot to write the AMP step down
how goondu
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